CRISPR and RNAi Systems

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  • Publisher : Elsevier
  • Release : 27 February 2021
  • ISBN : 9780128219119
  • Page : 840 pages
  • Rating : 4.5/5 from 103 voters

CRISPR and RNAi Systems Book PDF summary

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

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CRISPR and RNAi Systems

CRISPR and RNAi Systems
  • Author : Kamel A. Abd-Elsalam,Ki-Taek Lim
  • Publisher : Elsevier
  • Release Date : 2021-02-27
  • ISBN : 9780128219119
DOWNLOAD BOOKCRISPR and RNAi Systems

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is

Modulating Gene Expression

Modulating Gene Expression
  • Author : Aditi Singh,Mohammad W. Khan
  • Publisher : BoD – Books on Demand
  • Release Date : 2019-05-29
  • ISBN : 9781838806965
DOWNLOAD BOOKModulating Gene Expression

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in

RNA Interference and CRISPR Technologies

RNA Interference and CRISPR Technologies
  • Author : Mouldy Sioud
  • Publisher : Unknown
  • Release Date : 2020
  • ISBN : 107160290X
DOWNLOAD BOOKRNA Interference and CRISPR Technologies

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies
  • Author : Mohammad W. Khan,Aditi Singh
  • Publisher : Unknown
  • Release Date : 2019
  • ISBN : 1838806989
DOWNLOAD BOOKModulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in

CRISPR-Cas Systems

CRISPR-Cas Systems
  • Author : Rodolphe Barrangou,John van der Oost
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-13
  • ISBN : 9783642346576
DOWNLOAD BOOKCRISPR-Cas Systems

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B
  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2021-04-29
  • ISBN : 9780323852982
DOWNLOAD BOOKReprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This

RNA-Based Technologies for Functional Genomics in Plants

RNA-Based Technologies for Functional Genomics in Plants
  • Author : Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh
  • Publisher : Springer Nature
  • Release Date : 2021-05-13
  • ISBN : 9783030649944
DOWNLOAD BOOKRNA-Based Technologies for Functional Genomics in Plants

This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for

Tomato Class II Glutaredoxin Mutants Generated Via Multiplex CRISPR/Cas9 Genome Editing Technology are Susceptible to Multiple Abiotic Stresses

Tomato Class II Glutaredoxin Mutants Generated Via Multiplex CRISPR/Cas9 Genome Editing Technology are Susceptible to Multiple Abiotic Stresses
  • Author : Tayebeh Kakeshpour
  • Publisher : Unknown
  • Release Date : 2020
  • ISBN : OCLC:1224537262
DOWNLOAD BOOKTomato Class II Glutaredoxin Mutants Generated Via Multiplex CRISPR/Cas9 Genome Editing Technology are Susceptible to Multiple Abiotic Stresses

Gene silencing technologies such as clustered regulatory short palindromic repeats (CRISPR)/Cas9 and RNA interference (RNAi) created a revolution in genome engineering. They are highly site-specific, simple, fast, and cost-effective. Since their discovery, gene silencing technologies have extensively been implemented in various organisms including humans, animals, plants, and microbes. They have been used for both basic science studies such as gene functional analysis and applied science such as medicine and crop improvement. In this work, we used multiplex CRISPR/Cas9

Diversity and Evolution of Butterfly Wing Patterns

Diversity and Evolution of Butterfly Wing Patterns
  • Author : Toshio Sekimura,H. Frederik Nijhout
  • Publisher : Springer
  • Release Date : 2017-08-29
  • ISBN : 9789811049569
DOWNLOAD BOOKDiversity and Evolution of Butterfly Wing Patterns

This book facilitates an integrative understanding of the development, genetics and evolution of butterfly wing patterns. To develop a deep and realistic understanding of the diversity and evolution of butterfly wing patterns, it is essential and necessary to approach the problem from various kinds of key research fields such as “evo-devo,” “eco-devo,” ”developmental genetics,” “ecology and adaptation,” “food plants,” and “theoretical modeling.” The past decade-and-a-half has seen a veritable revolution in our understanding of the development, genetics and evolution of

Biotechnologies of Crop Improvement, Volume 2

Biotechnologies of Crop Improvement, Volume 2
  • Author : Satbir Singh Gosal,Shabir Hussain Wani
  • Publisher : Springer
  • Release Date : 2018-07-09
  • ISBN : 9783319906508
DOWNLOAD BOOKBiotechnologies of Crop Improvement, Volume 2

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide

RNAi Technology

RNAi Technology
  • Author : R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
  • Publisher : CRC Press
  • Release Date : 2016-04-19
  • ISBN : 9781439873250
DOWNLOAD BOOKRNAi Technology

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

CRISPR-Cas in Agriculture: Opportunities and Challenges

CRISPR-Cas in Agriculture: Opportunities and Challenges
  • Author : Sandeep Kumar,Hiroshi Ezura,Vladimir Nekrasov,Linda Ann Rymarquis
  • Publisher : Frontiers Media SA
  • Release Date : 2021-05-28
  • ISBN : 9782889668151
DOWNLOAD BOOKCRISPR-Cas in Agriculture: Opportunities and Challenges

Mapping Genetic Interactions

Mapping Genetic Interactions
  • Author : Franco Joseph Vizeacoumar,Andrew Freywald
  • Publisher : Humana
  • Release Date : 2021-12-07
  • ISBN : 1071617397
DOWNLOAD BOOKMapping Genetic Interactions

This volume details methods of identifying synthetic lethal, genetic interactions by various approaches in different model systems including human cancer cells. Chapters guide readers through genetic interactions in model organisms, RNA interference, CRISPR/Cas9 based genome editing technologies, drug-gene interactions, mapping chemical genetic interactions, synergistic drug-gene relations, single cell sequencing, gene expression profiling, and novel genetic interactions. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary

Regulatory Aspects of Gene Therapy and Cell Therapy Products

Regulatory Aspects of Gene Therapy and Cell Therapy Products
  • Author : Maria Cristina Galli,Mercedes Serabian
  • Publisher : Springer
  • Release Date : 2015-09-15
  • ISBN : 9783319186184
DOWNLOAD BOOKRegulatory Aspects of Gene Therapy and Cell Therapy Products

This book discusses the different regulatory pathways for gene therapy (GT) and cell therapy (CT) medicinal products implemented by national and international bodies throughout the world (e.g. North and South America, Europe, and Asia). Each chapter, authored by experts from various regulatory bodies throughout the international community, walks the reader through the applications of nonclinical research to translational clinical research to licensure for these innovative products. More specifically, each chapter offers insights into fundamental considerations that are essential for

Biodefense in the Age of Synthetic Biology

Biodefense in the Age of Synthetic Biology
  • Author : National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Life Sciences,Board on Chemical Sciences and Technology,Committee on Strategies for Identifying and Addressing Potential Biodefense Vulnerabilities Posed by Synthetic Biology
  • Publisher : National Academies Press
  • Release Date : 2018-12-05
  • ISBN : 9780309465212
DOWNLOAD BOOKBiodefense in the Age of Synthetic Biology

Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise,